Title: trans-Golgi retention of a plasma membrane protein: mutations in the cytoplasmic domain of the asialoglycoprotein receptor subunit H1 result in trans-Golgi retention Document date: 1995_7_2
ID: tedj3xxz_27
Snippet: to the result obtained after similar treatment of the hepatoma cell line HepG2 which expresses the native ASGP receptor (lanes 1 and 2). Hl(A4-33A), however, was completely protected (lanes 7 and 8). During a 30-rain incubation at 37°C with proteinase K, all complex H1 (97%) was digested (lanes 3 and 6), reflecting surface appearance of initially intracellular protein. In contrast, Hl(A4-33A) was 100% protected even at 37°C, indicating that the.....
Document: to the result obtained after similar treatment of the hepatoma cell line HepG2 which expresses the native ASGP receptor (lanes 1 and 2). Hl(A4-33A), however, was completely protected (lanes 7 and 8). During a 30-rain incubation at 37°C with proteinase K, all complex H1 (97%) was digested (lanes 3 and 6), reflecting surface appearance of initially intracellular protein. In contrast, Hl(A4-33A) was 100% protected even at 37°C, indicating that the mutant protein did not pass through the plasma membrane to a significant extent within 30 rain (lane 9).
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