Selected article for: "antibody fragment and expression vector"
Author: Kopertekh, Lilya; Meyer, Torsten; Freyer, Cornelia; Hust, Michael
Title: Transient plant production of Salmonella Typhimurium diagnostic antibodies
  • Document date: 2019_2_12
    • ID: y47ahl3p_26
    Snippet: To confirm that the elevated mRNA levels correlated with the increased protein accumulation, the levels of scFv-TM43-E10 and scFv-Fc-TM43-E10 antibody fragments were measured using ELISA in plant tissue. The pLH-gb-PVX-m expression vector resulted in higher protein yields in comparison to the pLH-PVX-m vector for both antibody formats. As illustrated in Fig. 3 about 41 mg/g fresh leaf weight for the pLH-PVX-m-scFv-TM43-E10 agroinfiltrated leaves .....
    Document: To confirm that the elevated mRNA levels correlated with the increased protein accumulation, the levels of scFv-TM43-E10 and scFv-Fc-TM43-E10 antibody fragments were measured using ELISA in plant tissue. The pLH-gb-PVX-m expression vector resulted in higher protein yields in comparison to the pLH-PVX-m vector for both antibody formats. As illustrated in Fig. 3 about 41 mg/g fresh leaf weight for the pLH-PVX-m-scFv-TM43-E10 agroinfiltrated leaves and 82.5 mg/g fresh weight for the pLH-gb-PVX-m-scFv-TM43-E10 samples were detected. The same tendency was observed for the scFv-Fc-TM43-E10 antibody fragment: pLH-gb-PVX-m-scFv-Fc-TM43-E10 yielded 9.7 mg/g fresh leaf weight compared to the pLH-PVX-m-scFv-Fc-TM43-E10, which yielded 45.9 mg/g fresh leaf weight.

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