Author: Mack, Ethan A.; Kallal, Lara E.; Demers, Delia A.; Biron, Christine A.
Title: Type 1 Interferon Induction of Natural Killer Cell Gamma Interferon Production for Defense during Lymphocytic Choriomeningitis Virus Infection Document date: 2011_8_9
ID: qkwo747o_31
Snippet: LCMV plaque assay. LCMV titers were determined as previously described (7, 17) . Briefly, peritoneal lavage fluids were thawed from storage at Ϫ80°C and serially diluted. Samples were overlaid onto Vero cell monolayers and incubated at 37°C for 1.5 h. Plaques were allowed to form upon overlay of samples with a 50:50 mixture of agarose-medium 199 (Sigma) for 3 days and then visualized using neutral red staining (Sigma). Included in each assay w.....
Document: LCMV plaque assay. LCMV titers were determined as previously described (7, 17) . Briefly, peritoneal lavage fluids were thawed from storage at Ϫ80°C and serially diluted. Samples were overlaid onto Vero cell monolayers and incubated at 37°C for 1.5 h. Plaques were allowed to form upon overlay of samples with a 50:50 mixture of agarose-medium 199 (Sigma) for 3 days and then visualized using neutral red staining (Sigma). Included in each assay were positive LCMV control samples and negative controls (uninfected animals).
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