Author: Sneha Rath; Eliza Prangley; Jesse Donovan; Kaitlin Demarest; Yigal Meir; Ned Wingreen; Alexei Korennykh
Title: Concerted 2-5A-Mediated mRNA Decay and Transcription Reprogram Protein Synthesis in dsRNA Response Document date: 2018_12_4
ID: ng5c7xai_43
Snippet: Human cells were grown in RPMI with 10% FBS. For poly I:C transfections, 1 ïg/mL poly I:C was transfected using Lipofectamine 2000 Reagent (Thermo Fisher Scientific) for the indicated durations. For experiments aimed at measuring mRNA decay rates, RNA polymerase II transcription was blocked by adding 1 ïg/ml actinomycin D directly to the cell culture medium for the indicated durations. To measure decay rates of poly I:C-induced transcripts, W.....
Document: Human cells were grown in RPMI with 10% FBS. For poly I:C transfections, 1 ïg/mL poly I:C was transfected using Lipofectamine 2000 Reagent (Thermo Fisher Scientific) for the indicated durations. For experiments aimed at measuring mRNA decay rates, RNA polymerase II transcription was blocked by adding 1 ïg/ml actinomycin D directly to the cell culture medium for the indicated durations. To measure decay rates of poly I:C-induced transcripts, WT and RNase L -/cells were treated with 1 ïg/ml poly I:C for 4 hours, followed by actinomycin D treatment.
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