Selected article for: "cell culture and room temperature"
Author: Wang, Yi; Liu, Li
Title: The Membrane Protein of Severe Acute Respiratory Syndrome Coronavirus Functions as a Novel Cytosolic Pathogen-Associated Molecular Pattern To Promote Beta Interferon Induction via a Toll-Like-Receptor-Related TRAF3-Independent Mechanism
  • Document date: 2016_2_9
    • ID: uf96jgig_27
    Snippet: Transient transfection. Cells (HEK293ET, HEK293T, and J2-M) were transiently transfected with the plasmid DNAs (pCMV-Myc-M, pCMV-Myc, pGL3-IFN-␤-luc, or pNF-B-luc) using VigoFect (Vigorous Biotechnology, Beijing, China) according to the manufacturer's instructions. For example, about 5 g plasmid DNAs was first added to 100 l of 0.9% NaCl. Then, 2.5 l VigoFect was resuspended into another 100 l of 0.9% NaCl solution. Then, the DNA-NaCl mixture w.....
    Document: Transient transfection. Cells (HEK293ET, HEK293T, and J2-M) were transiently transfected with the plasmid DNAs (pCMV-Myc-M, pCMV-Myc, pGL3-IFN-␤-luc, or pNF-B-luc) using VigoFect (Vigorous Biotechnology, Beijing, China) according to the manufacturer's instructions. For example, about 5 g plasmid DNAs was first added to 100 l of 0.9% NaCl. Then, 2.5 l VigoFect was resuspended into another 100 l of 0.9% NaCl solution. Then, the DNA-NaCl mixture was added to the VigoFect-NaCl mixture drop by drop with gentle vortexing. After a 15min incubation at room temperature, the reaction product was evenly distributed onto the cell culture surface of either 6-well plates or 35-mm 2 dishes and then continuously incubated for 48 h before harvesting.

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