Author: Wang, Yi; Liu, Li
Title: The Membrane Protein of Severe Acute Respiratory Syndrome Coronavirus Functions as a Novel Cytosolic Pathogen-Associated Molecular Pattern To Promote Beta Interferon Induction via a Toll-Like-Receptor-Related TRAF3-Independent Mechanism Document date: 2016_2_9
ID: uf96jgig_28
Snippet: Construction of the 293 siTRAF3 stable cells. Plasmid pSilencer-siTRAF3 DNAs (5 g) were transfected into HEK293 cells. After a 24-h transfection, the cell culture medium was replaced with fresh medium containing 1,200 g/ml G418. After 2 weeks of culture, cell colonies were picked up and expanded in a 24-well tissue culture plate. Finally, Western blot analysis was performed to detect TRAF3 expression......
Document: Construction of the 293 siTRAF3 stable cells. Plasmid pSilencer-siTRAF3 DNAs (5 g) were transfected into HEK293 cells. After a 24-h transfection, the cell culture medium was replaced with fresh medium containing 1,200 g/ml G418. After 2 weeks of culture, cell colonies were picked up and expanded in a 24-well tissue culture plate. Finally, Western blot analysis was performed to detect TRAF3 expression.
Search related documents:
Co phrase search for related documents- blot analysis and cell culture: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20
- blot analysis and cell culture medium: 1, 2
- blot analysis and fresh medium: 1, 2
- blot analysis and stable cell: 1, 2, 3, 4, 5, 6, 7, 8
- blot analysis and tissue culture: 1, 2, 3, 4, 5, 6
- blot analysis and western blot analysis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- cell colony and fresh medium: 1
- cell colony and stable cell: 1, 2, 3
- cell colony and western blot analysis: 1, 2, 3
- cell culture and fresh medium: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
- cell culture and stable cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- cell culture and tissue culture: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- cell culture and tissue culture plate: 1
- cell culture and western blot analysis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
- cell culture medium and fresh medium: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- cell culture medium and stable cell: 1, 2
- cell culture medium and tissue culture: 1, 2, 3, 4, 5, 6
- cell culture medium and western blot analysis: 1, 2
- fresh medium and western blot analysis: 1, 2
Co phrase search for related documents, hyperlinks ordered by date