Title: The organization of endoplasmic reticulum export complexes Document date: 1996_10_1
ID: xxlcdbqi_55
Snippet: To develop a three-dimensional view of export complexes, we applied for the first time a modification of the quickfreeze, deep-etch methodologies used previously to visualize vesicles budding from the plasma membrane (Heuser, 1980) and Golgi compartments (Weidman et al., 1993) . The approach is particularly applicable to semi-intact cells where the cytosol can be readily washed away to reveal structural features of the ER membrane surface......
Document: To develop a three-dimensional view of export complexes, we applied for the first time a modification of the quickfreeze, deep-etch methodologies used previously to visualize vesicles budding from the plasma membrane (Heuser, 1980) and Golgi compartments (Weidman et al., 1993) . The approach is particularly applicable to semi-intact cells where the cytosol can be readily washed away to reveal structural features of the ER membrane surface.
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