Author: Ishibashi, Daisuke; Homma, Takujiro; Nakagaki, Takehiro; Fuse, Takayuki; Sano, Kazunori; Satoh, Katsuya; Mori, Tsuyoshi; Atarashi, Ryuichiro; Nishida, Noriyuki
Title: Type I interferon protects neurons from prions in in vivo models Document date: 2019_2_7
ID: zopwlaq4_24
Snippet: In a fluorescence-activated cell sorter analysis, immortalized cells established from IFNAR1-deficient mouse embryonic fibroblasts (Ifnar1 À/À MEF) and the Ifnar1 À/À cells reintroduced with the MSCV retrovirus coded mouse Ifnar1 gene and overexpressing exogenous IFNAR1 (IFNAR1 Ifnar1 À/À ) were harvested in PBS containing 20 mM EDTA. Cells were suspended, fixed, and permeabilized with BD Cytofix/ Cytoperm TM Fixation/Permeabilization Solut.....
Document: In a fluorescence-activated cell sorter analysis, immortalized cells established from IFNAR1-deficient mouse embryonic fibroblasts (Ifnar1 À/À MEF) and the Ifnar1 À/À cells reintroduced with the MSCV retrovirus coded mouse Ifnar1 gene and overexpressing exogenous IFNAR1 (IFNAR1 Ifnar1 À/À ) were harvested in PBS containing 20 mM EDTA. Cells were suspended, fixed, and permeabilized with BD Cytofix/ Cytoperm TM Fixation/Permeabilization Solution Kit (BD Biosciences), and reacted with anti-SAF32 and MAR1-5A3 (1:100) as primary antibodies, and Alexa Fluor Õ 488 goat anti-rabbit and anti-mouse IgG (Molecular Probes, 1:100). The cells were analysed using a flow cytometer (BD FACSCalibur TM , BD Biosciences).
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