Author: Dawson, Patrick; Malik, Mamunur Rahman; Parvez, Faruque; Morse, Stephen S.
Title: What Have We Learned About Middle East Respiratory Syndrome Coronavirus Emergence in Humans? A Systematic Literature Review Document date: 2019_3_1
ID: yb54i1ne_25
Snippet: Reverse transcription PCR either real time or conventional, is now standard. For diagnostic screening, PCR primer sets to regions upstream of the E gene (''up-E'') are most frequently used, with ORF1a or ORF1b for confirmation (Corman et al. 2012a (Corman et al. , 2012b . Sensitivity was reported as *3.4 viral gene copies/25 lL reaction for up-E and *64 copies for ORF1b, with specificity approaching 100% using a test panel of other known coronavi.....
Document: Reverse transcription PCR either real time or conventional, is now standard. For diagnostic screening, PCR primer sets to regions upstream of the E gene (''up-E'') are most frequently used, with ORF1a or ORF1b for confirmation (Corman et al. 2012a (Corman et al. , 2012b . Sensitivity was reported as *3.4 viral gene copies/25 lL reaction for up-E and *64 copies for ORF1b, with specificity approaching 100% using a test panel of other known coronaviruses and clinical respiratory samples (Corman et al. 2012a ). ORF1a PCR was reported to have comparable sensitivity and specificity to up-E (Corman et al. 2012b) . Commercial kits are available, with comparable results reported (Corman et al. 2014c , Seong et al. 2016 , Mohamed et al. 2017 .
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