Title: Yeast Kex1p is a Golgi-associated membrane protein: deletions in a cytoplasmic targeting domain result in mislocalization to the vacuolar membrane Document date: 1992_12_2
ID: ucguzgdm_18
Snippet: YCp50-CT2 contains the ADH/promoter and multiple cloning site from the plasmid pVT-100-L (Veruet et al., 1987) cloned into the plnsmid YCpS0. The termini of the 3.0-kb BgllI-HindIII fragment of KEX/were filled with Klenow enzyme and inserted into YCp50-CT2 which had been digested with Pvull. The resulting plasmid, pKXI-24, results in an ~10-fold overproduction of Kexlp......
Document: YCp50-CT2 contains the ADH/promoter and multiple cloning site from the plasmid pVT-100-L (Veruet et al., 1987) cloned into the plnsmid YCpS0. The termini of the 3.0-kb BgllI-HindIII fragment of KEX/were filled with Klenow enzyme and inserted into YCp50-CT2 which had been digested with Pvull. The resulting plasmid, pKXI-24, results in an ~10-fold overproduction of Kexlp.
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