Selected article for: "negative sample and positive sample"
Author: Shefali Dobhal; Gamze Boluk; Brooke Babler; Michael J. Stulberg; John Rascoe; Mark Nakhla; Toni A. Chapman; Alex B. Crockford; Michael Melzer; Anne M. Alvarez; Mohammad Arif
Title: Comparative genomics reveals signature regions used to develop a robust and sensitive multiplex TaqMan real-time qPCR assay to detect the genus Dickeya and Dickeya dianthicola
  • Document date: 2019_11_20
    • ID: lgeu4id0_19
    Snippet: The developed TaqMan qPCR was used to screen the samples, collected from six potato growing states of USA, for the genus Dickeya and/or D. dianthicola contamination/infection. A total of 132 asymptomatic, symptomatic plant samples which included potato stems, tubers and roots, and 3 from potato fields water sources, were screened. The tests were performed at the Wisconsin Seed Potato Certification Laboratory (Wisconsin, USA). Each sample was repl.....
    Document: The developed TaqMan qPCR was used to screen the samples, collected from six potato growing states of USA, for the genus Dickeya and/or D. dianthicola contamination/infection. A total of 132 asymptomatic, symptomatic plant samples which included potato stems, tubers and roots, and 3 from potato fields water sources, were screened. The tests were performed at the Wisconsin Seed Potato Certification Laboratory (Wisconsin, USA). Each sample was replicated once; the positive and the negative template controls (NTC) were included in each test run.

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