Title: trans-Golgi retention of a plasma membrane protein: mutations in the cytoplasmic domain of the asialoglycoprotein receptor subunit H1 result in trans-Golgi retention Document date: 1995_7_2
ID: tedj3xxz_18
Snippet: [35S]Sulfate labeling was performed as described by Leitinger et al. (1994) . Cell lysates were immunoprecipitated with the polyclonal antiserum directed against the ASGP receptor and protein A-Sepharose (Pharmacia LKB Biotechnology, Piscataway, NJ). The immunocomplexes were solubilized in SDS-gel sample buffer and analyzed by gel electrophoresis and fluorography......
Document: [35S]Sulfate labeling was performed as described by Leitinger et al. (1994) . Cell lysates were immunoprecipitated with the polyclonal antiserum directed against the ASGP receptor and protein A-Sepharose (Pharmacia LKB Biotechnology, Piscataway, NJ). The immunocomplexes were solubilized in SDS-gel sample buffer and analyzed by gel electrophoresis and fluorography.
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