Author: Kopertekh, Lilya; Meyer, Torsten; Freyer, Cornelia; Hust, Michael
Title: Transient plant production of Salmonella Typhimurium diagnostic antibodies Document date: 2019_2_12
ID: y47ahl3p_25
Snippet: The scFv-TM43-E10 and scFv-Fc-TM43-E10 coding sequences were cloned into pLH-PVX-m and pLH-gb-PVX-m plasmids (Fig. 1) . The mRNA expression of target genes was studied in agroinfiltrated samples by qPCR using TM43-E10 specific primers and cyp gene as an internal control. Co-expression of gb silencing suppressor resulted in higher mRNA accumulation level by up to 4.1 and 17 fold for scFv-TM43-E10 and scFv-Fc-TM43-E10, respectively, when compared w.....
Document: The scFv-TM43-E10 and scFv-Fc-TM43-E10 coding sequences were cloned into pLH-PVX-m and pLH-gb-PVX-m plasmids (Fig. 1) . The mRNA expression of target genes was studied in agroinfiltrated samples by qPCR using TM43-E10 specific primers and cyp gene as an internal control. Co-expression of gb silencing suppressor resulted in higher mRNA accumulation level by up to 4.1 and 17 fold for scFv-TM43-E10 and scFv-Fc-TM43-E10, respectively, when compared with plants infiltrated with the pLH-PVX-m vector (Fig. 2a) . The expression of recombinant antibody fragments was verified at protein level by immunoblotting. This analysis demonstrated the presence of the protein bands of expected molecular size for scFv-TM43-E10 and scFv-Fc-TM43-E10 in leaf extracts (Fig. 2b) .
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