Selected article for: "Ct value and Real time"

Author: Shefali Dobhal; Gamze Boluk; Brooke Babler; Michael J. Stulberg; John Rascoe; Mark Nakhla; Toni A. Chapman; Alex B. Crockford; Michael Melzer; Anne M. Alvarez; Mohammad Arif
Title: Comparative genomics reveals signature regions used to develop a robust and sensitive multiplex TaqMan real-time qPCR assay to detect the genus Dickeya and Dickeya dianthicola
  • Document date: 2019_11_20
  • ID: lgeu4id0_33
    Snippet: The developed multiplex TaqMan real-time qPCR was used to screen 143 field samples (stem, potato tuber, root and water) for Dickeya and specifically for D. dianthicola infection ( Table 3) . Out of 143 samples, 61 were positive for genus Dickeya and of these, 60 were positive for D. dianthicola. Samples crossing the threshold value (Ct) after 35 cycles were considered negative......
    Document: The developed multiplex TaqMan real-time qPCR was used to screen 143 field samples (stem, potato tuber, root and water) for Dickeya and specifically for D. dianthicola infection ( Table 3) . Out of 143 samples, 61 were positive for genus Dickeya and of these, 60 were positive for D. dianthicola. Samples crossing the threshold value (Ct) after 35 cycles were considered negative.

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