Author: Mack, Ethan A.; Kallal, Lara E.; Demers, Delia A.; Biron, Christine A.
Title: Type 1 Interferon Induction of Natural Killer Cell Gamma Interferon Production for Defense during Lymphocytic Choriomeningitis Virus Infection Document date: 2011_8_9
ID: qkwo747o_29
Snippet: To ensure proper identification of the NK cell population in PECs, NK1.1-positive cells were costained with markers identifying TCR-â£/⤠and TCR-â¥/⦠T cells. A lymphocyte gate was set to maximize inclusion of NK1.1 Ï© cells at different times after infection, and it was identified as an "extended lymphocyte gate." Using this scheme, Ͻ5% of the NK1.1 Ï© cells were TCR-⦠ϩ under naive conditions; after LCMV infection, the population was .....
Document: To ensure proper identification of the NK cell population in PECs, NK1.1-positive cells were costained with markers identifying TCR-â£/⤠and TCR-â¥/⦠T cells. A lymphocyte gate was set to maximize inclusion of NK1.1 Ï© cells at different times after infection, and it was identified as an "extended lymphocyte gate." Using this scheme, Ͻ5% of the NK1.1 Ï© cells were TCR-⦠ϩ under naive conditions; after LCMV infection, the population was reduced to Ͻ2%, and the cells were not producing IFN-⥠(data not shown). NK1.1 Ï© cells that were TCR-⤠ϩ (NK T cells) represented approximately 30% of the total NK1.1 Ï© population at day 0. After LCMV infection, this population was again diminished to Ͻ5%, and the IFN-⥠produced by these cells represented only about 3% of the total IFN-⥠ϩ cells. Thus, all experiments defined NK cells to be NK1.1 Ï© TCR-⤠Ϫ .
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