Author: Hassanzad, Maryam; Nadji, Seyed Alireza; Darougar, Sepideh; Tashayoie-Nejad, Sabereh; Boloursaz, Mohammad Reza; Mahdaviani, Seyed Alireza; Baghaie, Nooshin; Ghaffaripour, Hosseinali; Velayati, Ali Akbar
Title: Association of specific viral infections with childhood asthma exacerbations Document date: 2018_9_27
ID: rqmjras4_11
Snippet: Influenza virus (types A and B) was detected by real-time PCR method based on WHO information for molecular diagnosis of influenza virus updated on March 2014 [9] . To screen for the human bocavirus (hBoV) genome, a nested PCR was performed using primers in the NS-1 coding region, as previously described [10] . Laboratory investigation of parainfluenzavirus types 1, 2, 3, 4; RSV A and B; HRVs; human coronaviruses OC43 and 229E; human metapneumovi.....
Document: Influenza virus (types A and B) was detected by real-time PCR method based on WHO information for molecular diagnosis of influenza virus updated on March 2014 [9] . To screen for the human bocavirus (hBoV) genome, a nested PCR was performed using primers in the NS-1 coding region, as previously described [10] . Laboratory investigation of parainfluenzavirus types 1, 2, 3, 4; RSV A and B; HRVs; human coronaviruses OC43 and 229E; human metapneumoviruses; and adenoviruses were conducted by multiplex nested PCR assays that were described earlier [11] . The positive clinical samples and the Vircella AmpliRun ® PCR control (Vircell, Spain) were used as positive controls in our assay.
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