ViralORFeome: an integrated database to generate a versatile collection of viral ORFs

Pellet, J.; Tafforeau, L.; Lucas-Hourani, M.; Navratil, V.; Meyniel, L.; Achaz, G.; Guironnet-Paquet, A.; Aublin-Gex, A.; Caignard, G.; Cassonnet, P.; Chaboud, A.; Chantier, T.; Deloire, A.; Demeret, C.; Le Breton, M.; Neveu, G.; Jacotot, L.; Vaglio, P.; Delmotte, S.; Gautier, C.; Combet, C.; Deleage, G.; Favre, M.; Tangy, F.; Jacob, Y.; Andre, P.; Lotteau, V.; Rabourdin-Combe, C.; Vidalain, P. O.

To validate 'entry' constructs by sequencing, ViralORFeome automatically aligns uploaded sequence traces onto virtual clones using either an extended Libalign Perl tool or Phred and T-Coffee programs that build first a contig of sequence traces before the alignment is performed (23, 24) . Validated 'entry' constructs can be subsequently used to recombine viral ORFs into various Gateway Õ -compatible destination vectors to achieve protein expression. ViralORFeome allows users to keep track of the different expression constructs that have been established for each viral ORF.