Author: Longhini, Andrew P.; LeBlanc, Regan M.; Becette, Owen; Salguero, Carolina; Wunderlich, Christoph H.; Johnson, Bruce A.; D'Souza, Victoria M.; Kreutz, Christoph; Dayie, T. Kwaku
Title: Chemo-enzymatic synthesis of site-specific isotopically labeled nucleotides for use in NMR resonance assignment, dynamics and structural characterizations Document date: 2016_4_7
ID: uhhtvdif_14
Snippet: NMR spectral processing was done in Topspin (Bruker Biospin) and NVFx (One Moon Scientific). Peak intensities were selected using in-house software by David Fushman. CPMG data were fit to a two-state exchange model using the full Bloch-McConnell matrix. The time-dependent evolution of magnetization during the CPMG period was solved numerically by non-linear least squares fitting using in-house Matlab software. Errors in fits were calculated using.....
Document: NMR spectral processing was done in Topspin (Bruker Biospin) and NVFx (One Moon Scientific). Peak intensities were selected using in-house software by David Fushman. CPMG data were fit to a two-state exchange model using the full Bloch-McConnell matrix. The time-dependent evolution of magnetization during the CPMG period was solved numerically by non-linear least squares fitting using in-house Matlab software. Errors in fits were calculated using Jacobian or 200 Monte Carlo simulations (33) , and the larger of the two errors was reported for CPMG and CEST relaxation dispersion analysis.
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