2017 ACVIM Forum Research Abstract Program

lammation models. This study investigated the effects of canine adipose tissue-derived (cAT)MSCs in a rat model of SAP induced by retrograde injection of 3% sodium taurocholate solution into the pancreatic duct. cATMSCs labeled with dioctadecyl-3,3,3 0 -tetramethylindo-carbocyanine perchlorate (1 9 10 7 cells/kg) were systemically administered to rats and pancreatic tissue was collected 3 days later for histopathological, quantitative real-time PCR, and immunocytochemical analyses. Greater numbers of infused cATMSCs were detected in the pancreas of SAP as compared to sham-operated rats. cATMSC infusion reduced pancreatic edema, inflammatory cell infiltration, and acinar cell necrosis, and decreased pancreatic expression of the pro-inflammatory cytokines tumor necrosis factor-a, interleukin (IL)-1b, -6, -12, -17, and -23 and interferon-c, while stimulating expression of the antiinflammatory cytokines IL-4 and IL-10 in SAP rats. Moreover, cATMSCs decreased the number of cluster of differentiation 3positive T cells and increased that of Forkhead box P3-positive T cells in the injured pancreas. These results indicate that cATMSCs can be effective as a cell-based therapeutic strategy for treatment of SAP in dogs. There is limited information regarding the likelihood and outcome of esophageal perforation secondary to an esophageal foreign body (EFB) in dogs.