Title: 2017 ACVIM Forum Research Abstract Program Document date: 2017_6_15
ID: ri2w5iby_425
Snippet: Data obtained from 13 healthy dogs demonstrated acceptable intra-assay CV for WB (2.5%, 0.3-10.8% (median, range)) and plasma (5.7%, 0.0-11.1%) and acceptable inter-assay CV for WB (11.1%, 3.9-21.2%) but not for plasma (24.2%, 4.1-105.0%). Plasma PMP concentrations equaled the noise level of negative controls, and antibody centrifugation (plasma protocol) markedly reduced this noise with a DL of 1560 PMP/lL. Antibody centrifugation was implemente.....
Document: Data obtained from 13 healthy dogs demonstrated acceptable intra-assay CV for WB (2.5%, 0.3-10.8% (median, range)) and plasma (5.7%, 0.0-11.1%) and acceptable inter-assay CV for WB (11.1%, 3.9-21.2%) but not for plasma (24.2%, 4.1-105.0%). Plasma PMP concentrations equaled the noise level of negative controls, and antibody centrifugation (plasma protocol) markedly reduced this noise with a DL of 1560 PMP/lL. Antibody centrifugation was implemented in another seven dogs. The results demonstrated acceptable WB intra-assay (2.4%, 1.2-6.7%)and interassay (6.0%, 0.0-12.3%) CV, except in one dog (58.2% inter-assay CV). Unfortunately, hemolysis and/or lipidemia interfered with plasma PMP quantification in five of the seven dogs.
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