Selected article for: "chain reaction and polymerase culture chain reaction"
Author: Viitanen, S.J.; Lappalainen, A.; Rajamäki, M.M.
Title: Co-infections with Respiratory Viruses in Dogs with Bacterial Pneumonia
  • Document date: 2015_3_27
    • ID: qkw27nfk_31
    Snippet: Bordetella bronchiseptica and Mycoplasma spp. were tested using both PCR and conventional culture methods. Polymerase chain reaction was, as expected, able to reliably demonstrate both pathogens in dogs with positive culture results. Additionally, Mycoplasma PCR was positive in 3 dogs with negative culture results. The clinical relevance of these positive results is difficult to interpret, because Mycoplasma spp. are also encountered in the respi.....
    Document: Bordetella bronchiseptica and Mycoplasma spp. were tested using both PCR and conventional culture methods. Polymerase chain reaction was, as expected, able to reliably demonstrate both pathogens in dogs with positive culture results. Additionally, Mycoplasma PCR was positive in 3 dogs with negative culture results. The clinical relevance of these positive results is difficult to interpret, because Mycoplasma spp. are also encountered in the respiratory tract of healthy dogs. 64 On the other hand, because Mycoplasma requires special culture methods (and in this study also shipping to an outside laboratory), there might have been dogs in which Mycoplasma culture was falsely negative. Quantitative PCR might have aided in assessing the clinical relevance of these PCR findings.

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