Selected article for: "RIPA buffer and Sigma RIPA buffer"
Author: Paquin, Ashley; Onabajo, Olusegun O.; Tang, Wei; Prokunina-Olsson, Ludmila
Title: Comparative Functional Analysis of 12 Mammalian IFN-?4 Orthologs
  • Document date: 2016_1_1
    • ID: sqxrwgif_19
    Snippet: After transfection for 48 h in 6-well plates at a density of 350,000 cells/well, cells were lysed with 350 mL of RIPA buffer (Sigma) supplemented with Complete Ultra protease inhibitor (Roche). Equal amounts of proteins were resolved on 4%-12% Bis-Tris Bolt gels and transferred using iBlot2 (Life Technologies). Detection was done using primary antibodies MAB-IFN-l4 (2 mg/mL), RAB-IFN-l4 (0.5 mg/ mL), and mouse a-Halo (1 mg/mL, G9211; Promega). Se.....
    Document: After transfection for 48 h in 6-well plates at a density of 350,000 cells/well, cells were lysed with 350 mL of RIPA buffer (Sigma) supplemented with Complete Ultra protease inhibitor (Roche). Equal amounts of proteins were resolved on 4%-12% Bis-Tris Bolt gels and transferred using iBlot2 (Life Technologies). Detection was done using primary antibodies MAB-IFN-l4 (2 mg/mL), RAB-IFN-l4 (0.5 mg/ mL), and mouse a-Halo (1 mg/mL, G9211; Promega). Secondary HRP-tagged goat anti-rabbit (sc-2030) or goat antimouse (sc-2031) antibodies from Santa Cruz were used at a 1:10,000 dilution. Signals were detected with HyGlo Quick Spray (Denville Scientific) and viewed on the ChemiDoc Touch imager with ImageLab 5.2 software (BioRad).

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