Author: Paquin, Ashley; Onabajo, Olusegun O.; Tang, Wei; Prokunina-Olsson, Ludmila
Title: Comparative Functional Analysis of 12 Mammalian IFN-?4 Orthologs Document date: 2016_1_1
ID: sqxrwgif_9
Snippet: The human hepatoma cell line, HepG2 (ATCC HB-8065), was purchased from the American Tissue Culture Collection (ATCC) and maintained in DMEM supplemented with 10% heat-inactivated fetal bovine serum (FBS). The custom ISRE-Luc-HepG2 cell line stably expressing the luciferase reporter under control of the interferon-stimulated response element (ISRE) has previously been described (Prokunina-Olsson and others 2013); the cells were maintained in DMEM .....
Document: The human hepatoma cell line, HepG2 (ATCC HB-8065), was purchased from the American Tissue Culture Collection (ATCC) and maintained in DMEM supplemented with 10% heat-inactivated fetal bovine serum (FBS). The custom ISRE-Luc-HepG2 cell line stably expressing the luciferase reporter under control of the interferon-stimulated response element (ISRE) has previously been described (Prokunina-Olsson and others 2013); the cells were maintained in DMEM supplemented with 10% FBS and 1 mg/mL of puromycin (Gold Biotechnology).
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