Author: Al-Mulla, Hawaa M. N.; Turrell, Lauren; Smith, Nicola M.; Payne, Luke; Baliji, Surendranath; Züst, Roland; Thiel, Volker; Baker, Susan C.; Siddell, Stuart G.; Neuman, Benjamin W.
Title: Competitive Fitness in Coronaviruses Is Not Correlated with Size or Number of Double-Membrane Vesicles under Reduced-Temperature Growth Conditions Document date: 2014_4_1
ID: tfuupgkg_16
Snippet: It has been previously shown that wild-type MHV produces about twice as much viral RNA at 40°C as at 33°C (23) (Fig. 2C ). To test whether this increased RNA synthesis was reflected in the number or size of replicative organelles, cells infected with wildtype virus and incubated at 40°C and 33°C were examined. Organelles formed at 40°C were smaller (Table 2 ) but more numerous (31 per cell compared to 18 per cell at 33°C), effectively doubl.....
Document: It has been previously shown that wild-type MHV produces about twice as much viral RNA at 40°C as at 33°C (23) (Fig. 2C ). To test whether this increased RNA synthesis was reflected in the number or size of replicative organelles, cells infected with wildtype virus and incubated at 40°C and 33°C were examined. Organelles formed at 40°C were smaller (Table 2 ) but more numerous (31 per cell compared to 18 per cell at 33°C), effectively doubling DMV capacity (Fig. 4A) . These results demonstrated that condi-tions reported to increase RNA yield correlated with increased DMV capacity for wild-type MHV.
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