Conflicts with diarrheal pathogens trigger rapid evolution of an intestinal signaling axis

Clayton M. Carey; Sarah E. Apple; Zoe A. Hilbert; Michael S. Kay; Nels C. Elde

. CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.29.014761 doi: bioRxiv preprint Figure S5 : Synthesis and oxidation of active STa peptides. (A) HPLC traces of each synthesized peptide shows evidence of ~8 peaks following air oxidation. Each peak was then collected for downstream analysis. (B) HEK293T cells expressing human GC-C were stimulated with the major purification products to identify the most potent fractions by measuring intracellular cGMP. For each peptide, the predominant oxidation product represented the most potent GC-C ligand. Some peaks were unable to be purified in isolation. (C) Final HPLC purification of toxin peptides used for analysis.