Title: Constitutive and basal secretion from the endocrine cell line, AtT-20 Document date: 1991_3_1
ID: tqgsavnr_19
Snippet: Anti-ACTH immunofluorescence was performed as described (Matsuuchi et al., 1988) . Cells were grown on poly-D-lysine-coated glass coverslips, incubated with rabbit anti-ACTH antibodies and sheep anti-rabbit IgG coupled to fluorescein (Cappel Laboratories, Cochranville, PA). Antisynaptophysin (p38) immunofluorescence was done on fixed and 0.01% Triton X-100 permeabilized cells with the monoclonal antibody, SY38 (Boehringer Mannheim Biochemicals) d.....
Document: Anti-ACTH immunofluorescence was performed as described (Matsuuchi et al., 1988) . Cells were grown on poly-D-lysine-coated glass coverslips, incubated with rabbit anti-ACTH antibodies and sheep anti-rabbit IgG coupled to fluorescein (Cappel Laboratories, Cochranville, PA). Antisynaptophysin (p38) immunofluorescence was done on fixed and 0.01% Triton X-100 permeabilized cells with the monoclonal antibody, SY38 (Boehringer Mannheim Biochemicals) diluted 1:2, followed by fluorescein-coupled goat anti-mouse ~/1 (diluted 1:10) as described (Matsunchi et al., 1988) . EM was performed as described (Matsuuchi et al., 1988) .
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