Author: Hasegawa, Daisuke; Ohnishi, Yumi; Koyama, Eiji; Matsunaga, Satoru; Ohtani, Shouhei; Nakanishi, Akio; Shiga, Takanori; Chambers, James K.; Uchida, Kazuyuki; Yokoi, Norihiko; Fukata, Yuko; Fukata, Masaki
Title: Deleted in colorectal cancer (netrin-1 receptor) antibodies and limbic encephalitis in a cat with hippocampal necrosis Document date: 2019_4_3
ID: zin6j6dc_4
Snippet: LGI1 autoantibodies are frequently found in humans and cats with LE. [5] [6] [7] [8] [9] The case cat serum antibodies as well as control (healthy and epileptic) cats serum antibodies (diluted 1 : 500) nonspecifically bound to COS7 (a common cell line originated from an African green monkey kidney) cell-surface slightly, irrespective of the expression of the LGI1 Then, a cell-surface binding assay using COS7 cells transfected with DCC was perform.....
Document: LGI1 autoantibodies are frequently found in humans and cats with LE. [5] [6] [7] [8] [9] The case cat serum antibodies as well as control (healthy and epileptic) cats serum antibodies (diluted 1 : 500) nonspecifically bound to COS7 (a common cell line originated from an African green monkey kidney) cell-surface slightly, irrespective of the expression of the LGI1 Then, a cell-surface binding assay using COS7 cells transfected with DCC was performed. Human neuromyotonia patient serum, used as a positive control, 8 bound to the DCC-expressing cells. The case cat serum showed a specific and strong binding to DCC-expressing cells ( Figure 6 ).
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