Deletions into an NH2-terminal hydrophobic domain result in secretion of rotavirus VP7, a resident endoplasmic reticulum membrane glycoprotein

For those cells treated with tunicamycin, dishes were incubated in medium containing tunicamycin (Sigma Chemical Co.), at a final concentration of 2 In the complete VP7 clone used for these studies, two conservative amino acid changes occurred compared with the originally published sequence derived from an incomplete clone (7) . C32 and L~7 are both changed to F. #g/ml (41) , beginning at 42.5 h after transfection and continuing for 8 h thereafter. Tunicamycin was therefore present for 4 h prior to radiolabeling, including the l-h preincubation in medium lacking methionine, as well as during the 4-h labeling period.