Author: Zhang, XiZhen; Wang, XiaoDan; Zhao, DongHai; Meng, XiangYu; Zhao, XingHong; Yu, XiangHui; Kong, Wei
Title: Design and immunogenicity assessment of HIV-1 virus-like particles as a candidate vaccine Document date: 2011_12_16
ID: xxypq3wd_25
Snippet: We investigated the expression stability in the stable cell line. Screened cell lines were maintained continuously in medium with G418. Expression of HIV-1 gagpol, and env protein were examined in cell lysate one month and 12 months after transfection, respectively. Efficient expression of the target protein could still be detected in the cell lines after 12 months. HIV-1 VLPs could also be detected in cellular supernatants of stable cell lines (.....
Document: We investigated the expression stability in the stable cell line. Screened cell lines were maintained continuously in medium with G418. Expression of HIV-1 gagpol, and env protein were examined in cell lysate one month and 12 months after transfection, respectively. Efficient expression of the target protein could still be detected in the cell lines after 12 months. HIV-1 VLPs could also be detected in cellular supernatants of stable cell lines ( Figure 2 ).
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