Author: Evonuk, Kirsten S.; Moseley, Carson E.; Doyle, Ryan E.; Weaver, Casey T.; DeSilva, Tara M.
Title: Determining Immune System Suppression versus CNS Protection for Pharmacological Interventions in Autoimmune Demyelination Document date: 2016_9_12
ID: vr83284f_8
Snippet: 4. Quantification of MBP staining by optical density 1. Open image and draw an area of interest as described in step 4.3.1. Go to Analyze > Set Measurements… and select "Mean gray value" (the sum of gray values within the selection divided by the number of pixels). Ensure that "Limit to threshold" is unchecked and "Display label" is checked. Click "OK" when complete. 2. To obtain measurements, go to Analyze > Measure. Observe a "Results" popup .....
Document: 4. Quantification of MBP staining by optical density 1. Open image and draw an area of interest as described in step 4.3.1. Go to Analyze > Set Measurements… and select "Mean gray value" (the sum of gray values within the selection divided by the number of pixels). Ensure that "Limit to threshold" is unchecked and "Display label" is checked. Click "OK" when complete. 2. To obtain measurements, go to Analyze > Measure. Observe a "Results" popup box appear. Copy this data and save as is or copy into another program. 3. For analysis, copy and paste values into another program. Convert mean gray value into optical density (OD) using the formula: OD = log 10 (255/mean gray value).
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