Selected article for: "nucleotide sequence and sequence alignment"
Author: PIEWBANG, Chutchai; RUNGSIPIPAT, Anudep; POOVORAWAN, Yong; TECHANGAMSUWAN, Somporn
Title: Development and application of multiplex PCR assays for detection of virus-induced respiratory disease complex in dogs
  • Document date: 2016_9_15
    • ID: w3g6ohac_11
    Snippet: Sequencing of PCR amplicons was performed to confirm their correct identity and thus the specificity of the PCR reaction. Amplicons were purified with a NucleoSpin Extract II (Macherey-Nagel, Düren, Germany) kit and submitted to The 1st BASE, Pte. Ltd. (Singapore) for direct sequencing. The derived nucleotide sequences were aligned using the BioEdit Sequence Alignment Editor version 7.0.9.0 software, and the respective consensus sequences were c.....
    Document: Sequencing of PCR amplicons was performed to confirm their correct identity and thus the specificity of the PCR reaction. Amplicons were purified with a NucleoSpin Extract II (Macherey-Nagel, Düren, Germany) kit and submitted to The 1st BASE, Pte. Ltd. (Singapore) for direct sequencing. The derived nucleotide sequences were aligned using the BioEdit Sequence Alignment Editor version 7.0.9.0 software, and the respective consensus sequences were compared to those in the GenBank database using BLASTn analysis.

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