Author: PIEWBANG, Chutchai; RUNGSIPIPAT, Anudep; POOVORAWAN, Yong; TECHANGAMSUWAN, Somporn
Title: Development and application of multiplex PCR assays for detection of virus-induced respiratory disease complex in dogs Document date: 2016_9_15
ID: w3g6ohac_22
Snippet: Evaluation of the multiplex PCR using clinical specimens: The multiplex PCRs were tested on the 51 NS and 51 OS samples (Fig. 3) and compared with the simplex PCR assays for each respective virus ( Table 2 ). The CAdV-2 and CRCoV detection had 100% sensitivity and specificity for both the NS and OS sampling sites. False negative results were observed in CaHV-1, CIV, CPIV and CDV detection when performing multiplex PCRs, which resulted in a lower .....
Document: Evaluation of the multiplex PCR using clinical specimens: The multiplex PCRs were tested on the 51 NS and 51 OS samples (Fig. 3) and compared with the simplex PCR assays for each respective virus ( Table 2 ). The CAdV-2 and CRCoV detection had 100% sensitivity and specificity for both the NS and OS sampling sites. False negative results were observed in CaHV-1, CIV, CPIV and CDV detection when performing multiplex PCRs, which resulted in a lower sensitivity of 87.5-97.7%. The PPV (100%) of all multiplex PCRs was consistent with the specificity (100%), while the NPV (89.5-99.0%) of those reactions was contrary with their sensitivity. Neither the multiplex RT-PCR nor the multiplex PCR showed false positive results when compared with its simplex counterpart.
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