Author: Kim, Yeong Hoon; Lee, Jihoo; Kim, Young-Eun; Chong, Chom-Kyu; Pinchemel, Yanaihara; Reisdörfer, Francis; Coelho, Joyce Brito; Dias, Ronaldo Ferreira; Bae, Pan Kee; Gusmão, Zuinara Pereira Maia; Ahn, Hye-Jin; Nam, Ho-Woo
Title: Development of a Rapid Diagnostic Test Kit to Detect IgG/IgM Antibody against Zika Virus Using Monoclonal Antibodies to the Envelope and Non-structural Protein 1 of the Virus Document date: 2018_2_28
ID: skzidybm_21
Snippet: RNA extracts from serum samples were prepared for PCR using an ExiPrep Viral RNA Kit (Bioneer Co., Daejeon, Korea). Viral RNA was extracted from 20 μl of serum and eluted in 50 μl of elution buffer. For the AccuPower ZIKV Multiplex Real-time RT-PCR assay, 5 μl of RNA extract was mixed with 45 μl of master mix, and real-time RT-PCR was performed using a PCR thermocycler (Exicycler 96 Real-Time Quantitative Thermal Block; Bioneer Co., Korea). A.....
Document: RNA extracts from serum samples were prepared for PCR using an ExiPrep Viral RNA Kit (Bioneer Co., Daejeon, Korea). Viral RNA was extracted from 20 μl of serum and eluted in 50 μl of elution buffer. For the AccuPower ZIKV Multiplex Real-time RT-PCR assay, 5 μl of RNA extract was mixed with 45 μl of master mix, and real-time RT-PCR was performed using a PCR thermocycler (Exicycler 96 Real-Time Quantitative Thermal Block; Bioneer Co., Korea). All of the procedures were performed according to the manufacturer's instructions.
Search related documents:
Co phrase search for related documents- Multiplex real time RT PCR assay and real time RT PCR assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30
- PCR thermocycler and Real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- PCR thermocycler and RNA extract: 1
- PCR thermocycler and rna kit: 1, 2, 3
- PCR thermocycler and RT PCR assay: 1
- PCR thermocycler and Viral rna kit: 1
- Real time and RNA extract: 1, 2, 3, 4, 5, 6, 7, 8
- Real time and rna kit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79
- Real time and RT PCR assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80
- Real time and serum sample: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- Real time and Viral rna kit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31
- real time RT PCR assay and RNA extract: 1
- real time RT PCR assay and rna kit: 1, 2, 3, 4, 5
- real time RT PCR assay and RT PCR assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79
- RNA extract and RT PCR assay: 1, 2, 3
- RNA extract and Viral rna kit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- rna kit and RT PCR assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- rna kit and Viral rna kit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82
- RT PCR assay and Viral rna kit: 1, 2, 3, 4, 5
Co phrase search for related documents, hyperlinks ordered by date