Author: Kim, Yeong Hoon; Lee, Jihoo; Kim, Young-Eun; Chong, Chom-Kyu; Pinchemel, Yanaihara; Reisdörfer, Francis; Coelho, Joyce Brito; Dias, Ronaldo Ferreira; Bae, Pan Kee; Gusmão, Zuinara Pereira Maia; Ahn, Hye-Jin; Nam, Ho-Woo
Title: Development of a Rapid Diagnostic Test Kit to Detect IgG/IgM Antibody against Zika Virus Using Monoclonal Antibodies to the Envelope and Non-structural Protein 1 of the Virus Document date: 2018_2_28
ID: skzidybm_27
Snippet: Construction of a highly specific RDT kit ZIKV E and NS1 proteins were exposed to the surface of the virus to be candidate targets as antigens. DNA sequences for the E and NS1 each were obtained from the Swiss Institute of Bioinformatics (http://viralzone.expasy.org) and cloned for the 261 amino acids of E and 354 amino acids of NS1 proteins. These 2 sequences are located adjacent to each other in the genome. Using these sequences, the ZIKV prote.....
Document: Construction of a highly specific RDT kit ZIKV E and NS1 proteins were exposed to the surface of the virus to be candidate targets as antigens. DNA sequences for the E and NS1 each were obtained from the Swiss Institute of Bioinformatics (http://viralzone.expasy.org) and cloned for the 261 amino acids of E and 354 amino acids of NS1 proteins. These 2 sequences are located adjacent to each other in the genome. Using these sequences, the ZIKV proteins were successfully expressed in Sf9 cells and purified as 37 and 45 kDa of molecular weight (Fig. 3) .
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