Author: Falzarano, Darryl; Kamissoko, Badian; de Wit, Emmie; Maïga, Ousmane; Cronin, Jacqueline; Samaké, Kassim; Traoré, Abdalah; Milne-Price, Shauna; Munster, Vincent J.; Sogoba, Nafomon; Niang, Mamadou; Safronetz, David; Feldmann, Heinz
Title: Dromedary camels in northern Mali have high seropositivity to MERS-CoV Document date: 2017_3_10
ID: rxba2p1s_5
Snippet: Serum for microneutralization assays was aliquoted in Mali and imported into the United States via the USDA (Plum Island, NY) where it was inactivated by gamma irradiation. 100 TCID 50 of MERS-CoV were added to two-fold dilutions of serum and incubated for 1 h at 37°C. The resulting mixture was added to Vero cells and incubated for 5 days when wells were scored for cytopathic effect. The virus neutralization titer was expressed as the reciprocal.....
Document: Serum for microneutralization assays was aliquoted in Mali and imported into the United States via the USDA (Plum Island, NY) where it was inactivated by gamma irradiation. 100 TCID 50 of MERS-CoV were added to two-fold dilutions of serum and incubated for 1 h at 37°C. The resulting mixture was added to Vero cells and incubated for 5 days when wells were scored for cytopathic effect. The virus neutralization titer was expressed as the reciprocal value of the highest dilution of serum that inhibits virus replication.
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