Author: Fang, H; Liu, A; Dahmen, U; Dirsch, O
Title: Dual role of chloroquine in liver ischemia reperfusion injury: reduction of liver damage in early phase, but aggravation in late phase Document date: 2013_6_27
ID: qmut89kb_28
Snippet: Quantitative polymerase chain reaction (PCR). Total RNA was extracted from liver tissue using the RNeasy kit (Qiagen, #74106, Hilden, Germany) and was reverse-transcribed into complementary DNA (cDNA) using the First-Strand cDNA synthesis kit (Invitrogen, #18080051, Carlsbad, CA, USA). Primers and probes ( Statistical analysis. Data were expressed as means±S.D. Differences between groups were evaluated for significance by one-way ANOVA analysis......
Document: Quantitative polymerase chain reaction (PCR). Total RNA was extracted from liver tissue using the RNeasy kit (Qiagen, #74106, Hilden, Germany) and was reverse-transcribed into complementary DNA (cDNA) using the First-Strand cDNA synthesis kit (Invitrogen, #18080051, Carlsbad, CA, USA). Primers and probes ( Statistical analysis. Data were expressed as means±S.D. Differences between groups were evaluated for significance by one-way ANOVA analysis. All tests were performed using SigmaStat v3.5 (Systat-Software, Erkrath, Germany). A P-value below 0.05 was considered statistically significant.
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