Author: Gröner, Albrecht; Broumis, Connie; Fang, Randel; Nowak, Thomas; Popp, Birgit; Schäfer, Wolfram; Roth, Nathan J.
Title: Effective inactivation of a wide range of viruses by pasteurization Document date: 2017_11_16
ID: w19hl2vs_8
Snippet: The following viruses and respective cell lines (Table S1 , available as supporting information in the online version of this paper) were used in the pasteurization studies: human parvovirus B19 (B19V), UT7/epo-S1; bovine her- Virus spiking stocks were derived from virus master and working banks. The cell culture supernatants containing the virus harvest (commonly cell culture medium without fetal bovine serum) were collected once a cytopathic e.....
Document: The following viruses and respective cell lines (Table S1 , available as supporting information in the online version of this paper) were used in the pasteurization studies: human parvovirus B19 (B19V), UT7/epo-S1; bovine her- Virus spiking stocks were derived from virus master and working banks. The cell culture supernatants containing the virus harvest (commonly cell culture medium without fetal bovine serum) were collected once a cytopathic effect had been detected. The virus stocks were clarified by low-speed centrifugation to remove cell debris and aliquots were stored frozen at -708C or below. Where higher-titer virus stocks were required, further concentration methods such as gradient centrifugation were employed. An initial titer of 4.5 log or greater cell culture infectious dose 50% (CCID 50 )/mL for all viruses studied was attained after spiking product intermediates.
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