Author: Lin, Mingqun; Liu, Hongyan; Xiong, Qingming; Niu, Hua; Cheng, Zhihui; Yamamoto, Akitsugu; Rikihisa, Yasuko
Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase Document date: 2016_8_19
ID: x5y551c8_28
Snippet: In vitro affinity isolation showed that glutathione S-transferase (GST)-RAB5A fusion protein, but not GST, pulled down native Etf-1 from E. chaffeensis-infected THP-1 cell lysates (Fig. S12A ). In addition, recombinant Etf-1 interacted with endogenous RAB5, PIK3C3, and BECN1 in uninfected THP-1 cells (Fig. S12B) . These results suggest that E. chaffeensis inclusions recruit Etf-1, RAB5, and major components of the class III PtdIns3K complex throu.....
Document: In vitro affinity isolation showed that glutathione S-transferase (GST)-RAB5A fusion protein, but not GST, pulled down native Etf-1 from E. chaffeensis-infected THP-1 cell lysates (Fig. S12A ). In addition, recombinant Etf-1 interacted with endogenous RAB5, PIK3C3, and BECN1 in uninfected THP-1 cells (Fig. S12B) . These results suggest that E. chaffeensis inclusions recruit Etf-1, RAB5, and major components of the class III PtdIns3K complex through protein-protein interactions.
Search related documents:
Co phrase search for related documents- affinity isolation and protein protein: 1
- cell lysate and fusion protein: 1, 2, 3, 4, 5, 6, 7
- cell lysate and GST fusion protein: 1, 2, 3
- cell lysate and protein protein: 1, 2, 3, 4, 5, 6, 7
- fusion protein and GST fusion protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40
- fusion protein and major component: 1
- fusion protein and protein protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81
- fusion protein and protein protein interaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
- fusion protein and uninfected cell: 1
- GST fusion protein and protein protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- major component and protein protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- major component and protein protein interaction: 1, 2
- protein protein and uninfected cell: 1, 2, 3, 4, 5, 6, 7, 8
Co phrase search for related documents, hyperlinks ordered by date