Selected article for: "fusion protein and GST protein"

Author: Lin, Mingqun; Liu, Hongyan; Xiong, Qingming; Niu, Hua; Cheng, Zhihui; Yamamoto, Akitsugu; Rikihisa, Yasuko
Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase
  • Document date: 2016_8_19
  • ID: x5y551c8_28
    Snippet: In vitro affinity isolation showed that glutathione S-transferase (GST)-RAB5A fusion protein, but not GST, pulled down native Etf-1 from E. chaffeensis-infected THP-1 cell lysates (Fig. S12A ). In addition, recombinant Etf-1 interacted with endogenous RAB5, PIK3C3, and BECN1 in uninfected THP-1 cells (Fig. S12B) . These results suggest that E. chaffeensis inclusions recruit Etf-1, RAB5, and major components of the class III PtdIns3K complex throu.....
    Document: In vitro affinity isolation showed that glutathione S-transferase (GST)-RAB5A fusion protein, but not GST, pulled down native Etf-1 from E. chaffeensis-infected THP-1 cell lysates (Fig. S12A ). In addition, recombinant Etf-1 interacted with endogenous RAB5, PIK3C3, and BECN1 in uninfected THP-1 cells (Fig. S12B) . These results suggest that E. chaffeensis inclusions recruit Etf-1, RAB5, and major components of the class III PtdIns3K complex through protein-protein interactions.

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