Author: Lin, Mingqun; Liu, Hongyan; Xiong, Qingming; Niu, Hua; Cheng, Zhihui; Yamamoto, Akitsugu; Rikihisa, Yasuko
Title: Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase Document date: 2016_8_19
ID: x5y551c8_61
Snippet: Uninfected or E. chaffeensis-infected THP-1 cells at 2 d p.i. were lysed in modified lysis buffer (25 mM Tris, pH 7.4, 150 mM NaCl, 1% NP40 [USB, 19638] , 5% glycerol, and 1% protease inhibitor cocktail III [Calbiochem, 539134]) for 15 min and immunoprecipitated for 2 h with rabbit anti-Etf-1, -BECN1, or -PIK3C3, or mouse anti-RAB5 IgG that was crosslinked to protein A/G-magnetic beads (Pierce, 88805). Normal rabbit (Santa Cruz Biotechnology, sc-.....
Document: Uninfected or E. chaffeensis-infected THP-1 cells at 2 d p.i. were lysed in modified lysis buffer (25 mM Tris, pH 7.4, 150 mM NaCl, 1% NP40 [USB, 19638] , 5% glycerol, and 1% protease inhibitor cocktail III [Calbiochem, 539134]) for 15 min and immunoprecipitated for 2 h with rabbit anti-Etf-1, -BECN1, or -PIK3C3, or mouse anti-RAB5 IgG that was crosslinked to protein A/G-magnetic beads (Pierce, 88805). Normal rabbit (Santa Cruz Biotechnology, sc-2027) or mouse IgG (Santa Cruz Biotechnology, sc-2025) was used as a negative control for immunoprecipitation. Bound proteins were eluted by 2£ SDS sample buffer and subjected to western blotting.
Search related documents:
Co phrase search for related documents- bind protein and protease inhibitor: 1
- bind protein and western blotting: 1, 2, 3, 4, 5, 6
- lysis buffer and modify lysis buffer: 1
- lysis buffer and negative control: 1, 2, 3
- lysis buffer and normal rabbit: 1
- lysis buffer and protease inhibitor: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- lysis buffer and sample buffer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- lysis buffer and SDS sample buffer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
- lysis buffer and western blotting: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23
- modify lysis buffer and sample buffer: 1
- negative control and normal rabbit: 1, 2, 3
- negative control and protease inhibitor: 1
- negative control and sample buffer: 1, 2, 3
- negative control and SDS sample buffer: 1
- negative control and western blotting: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
- protease inhibitor and sample buffer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
- protease inhibitor and SDS sample buffer: 1, 2, 3, 4, 5, 6, 7, 8
- protease inhibitor and western blotting: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- sample buffer and SDS sample buffer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
Co phrase search for related documents, hyperlinks ordered by date