Evaluation of an Immunochromatographic Assay for the Rapid and Simultaneous Detection of Rotavirus and Adenovirus in Stool Samples

Kim, Jayoung; Kim, Hyun Soo; Kim, Han-Sung; Kim, Jae-Seok; Song, Wonkeun; Lee, Kyu Man; Lee, Sunhwa; Park, Kyoung Un; Lee, Woochang; Hong, Young Jun

Interference tests were performed with the following substances: human blood, barium sulfate (contrast medium), loperamide (anti-diarrhea drug, Janssen, Seoul, Korea), metronidazole (antibiotics, CJ Pharma, Seoul, Korea), hemoglobin (Sigma-Aldrich Co., St. Louis, MO, USA), bilirubin (Sigma-Aldrich Co.), and triglyceride mix (Sigma-Aldrich Co.). Each substance (5 mg) was dissolved in 1 mL of solvent (hemoglobin in distilled water; triglyceride in ether; bilirubin in chloroform; all others in distilled water), and 50 μL of solution was mixed with both 950 μL of negative stool suspension (negative base pool) and 950 μL of low positive stool suspension (low positive base pool). The final concentrations of barium sulfate, loperamide, metronidazole, hemoglobin, bilirubin, and triglyceride mix for interference testing were 0.25 mg/mL each. For substances in the liquid form (e.g., blood), 50 μL of the substance was mixed with 950 μL of the negative and low positive base pool (1:20 dilution).