Author: Cha, Ran-hui; Yang, Seung Hee; Moon, Kyung Chul; Joh, Joon-Sung; Lee, Ji Yeon; Shin, Hyoung-Shik; Kim, Dong Ki; Kim, Yon Su
Title: A Case Report of a Middle East Respiratory Syndrome Survivor with Kidney Biopsy Results Document date: 2016_3_10
ID: rkwz1pwh_13
Snippet: The in situ detection of MERS-CoV was performed manually using a RNAscope kit (Advanced Cell Diagnostics, Hayward, CA, USA) according to the manufacturer's instructions. Briefly, 4-μm FFPE tissue sections were pretreated by heating and protease application prior to hybridization with a target probe to the MERS-CoV (MERS-CoV isolate England/2/2013 complete genome: 431631, Advanced Cell Diagnostics). Positive staining was indicated by the presence.....
Document: The in situ detection of MERS-CoV was performed manually using a RNAscope kit (Advanced Cell Diagnostics, Hayward, CA, USA) according to the manufacturer's instructions. Briefly, 4-μm FFPE tissue sections were pretreated by heating and protease application prior to hybridization with a target probe to the MERS-CoV (MERS-CoV isolate England/2/2013 complete genome: 431631, Advanced Cell Diagnostics). Positive staining was indicated by the presence of brown punctuate dots in the nucleus and/or cytoplasm. MERS-CoV expression was scored using the instructions for the RNAscope FFPE Assay Kit.
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