Author: Gaikwad, Satish S.; Lee, Hyun-Jeong; Kim, Ji-Ye; Choi, Kang-Seuk
Title: Expression and serological application of recombinant epitope-repeat protein carrying an immunodominant epitope of Newcastle disease virus nucleoprotein Document date: 2019_1_31
ID: tr3ageky_2
Snippet: The aim of the present study was to develop a serodiagnostic test for differentiation infected from vaccinated animal (DIVA) strategy accompanying an NDV marker vaccine lacking the NP IDE. For this purpose, an indirect enzyme-linked immunosorbent assay (ELISA) format employing a recombinant protein, which carries multimers of IDE, was developed. The recombinant protein was expressed in baculovirus system. Specificity and sensitivity of recombinan.....
Document: The aim of the present study was to develop a serodiagnostic test for differentiation infected from vaccinated animal (DIVA) strategy accompanying an NDV marker vaccine lacking the NP IDE. For this purpose, an indirect enzyme-linked immunosorbent assay (ELISA) format employing a recombinant protein, which carries multimers of IDE, was developed. The recombinant protein was expressed in baculovirus system. Specificity and sensitivity of recombinant protein was evaluated.
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