Author: Seong, Moon-Woo; Lee, Seung Jun; Cho, Sung Im; Ko, Kyungphil; Kim, Mi-Na; Sung, Heungsub; Kim, Jae-Seok; Ahn, Ji Soo; Yu, Byung Su; Kim, Taek Soo; Kim, Eui Chong; Park, Sung Sup
Title: External Quality Assessment of MERS-CoV Molecular Diagnostics During the 2015 Korean Outbreak Document date: 2015_2_23
ID: uiou7c2b_24
Snippet: In this survey, most participants used commercial rRT-PCR kits. Our results showed that significantly different CT values could be produced depending on the kit used. Though these kits are for semi-quantitative purposes and a difference in CT values does not correlate with differences in analytical sensitivity, a large difference in the CT values for the same material may influence the analytical sensitivity of the kit. Therefore, cut-off values .....
Document: In this survey, most participants used commercial rRT-PCR kits. Our results showed that significantly different CT values could be produced depending on the kit used. Though these kits are for semi-quantitative purposes and a difference in CT values does not correlate with differences in analytical sensitivity, a large difference in the CT values for the same material may influence the analytical sensitivity of the kit. Therefore, cut-off values should be carefully validated for each method in order to have optimal sensitivity for the detection of MERS-CoV in clinical settings. In addition, our study demonstrates that CT values might be different depending on the platform used for performing real-time PCR, even when using the same commercial kit. Therefore, the platform as well as the kit should be considered when establishing or validating the cut-off value of a kit. It is known that the analytical sensitivity of MERS-CoV RT-PCR depends on the target gene tested; the ORF1a assay has a lower sensitivity compared with the upE assay [3] . In this survey, all participants reported both upE and ORF1a results. Both assays gave comparable results, and no significant difference between them was observed.
Search related documents:
Co phrase search for related documents- low sensitivity and optimal sensitivity: 1, 2, 3, 4, 5, 6, 7
- low sensitivity and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
- low sensitivity and real time pcr: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57
- low sensitivity and real time pcr perform: 1
- low sensitivity and rrt pcr: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- low sensitivity and significant difference: 1, 2, 3, 4, 5, 6
- low sensitivity and target gene: 1, 2, 3, 4, 5
- real time and rrt pcr: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75
- real time and rrt pcr kit: 1, 2, 3, 4, 5
- real time and significant difference: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
- real time and significant difference observe: 1
- real time and target gene: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74
- real time and test target gene: 1, 2
- real time and upE assay: 1, 2, 3, 4, 5, 6, 7, 8, 9
- real time pcr and rrt pcr: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74
- real time pcr and rrt pcr kit: 1, 2, 3, 4, 5
- real time pcr and significant difference: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72
- real time pcr and significant difference observe: 1
- real time pcr and target gene: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74
Co phrase search for related documents, hyperlinks ordered by date