Author: Xu, Shengnan; Hu, Hai-Yu
Title: Fluorogen-activating proteins: beyond classical fluorescent proteins Document date: 2018_3_24
ID: sh3srp8g_10
Snippet: Cell surface proteins tagged with FAP are readily detected with cell membrane impermeable fluorogens, whereas proteins within the secretory compartments in the cell only could be stained with membrane permeable fluorogens. In order to increase or decrease cell membrane permeability, fluorogen 1-6 were developed based on MG or TO (Fig. 5) . With the intention of shorting the emission wavelength to blue, fluorogen 7 (MHN-ester) was explored 28 . MG.....
Document: Cell surface proteins tagged with FAP are readily detected with cell membrane impermeable fluorogens, whereas proteins within the secretory compartments in the cell only could be stained with membrane permeable fluorogens. In order to increase or decrease cell membrane permeability, fluorogen 1-6 were developed based on MG or TO (Fig. 5) . With the intention of shorting the emission wavelength to blue, fluorogen 7 (MHN-ester) was explored 28 . MG-ester 18, 29 and MHN-ester (fluorogen 1 and 7) showed good cell permeability. On the other hand, sulfonated groups, polyethelyene glycol groups and amino groups were used to reduce the cell membrane permeability, resulting in cell impermeable sulfonated fluorogens TO1, TO1-2p, polyethelyene glycol modified fluorogens MG-2p, MG-11p and sulfonated fluorogens MG-B-Tau 30 , respectively.
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