Author: Ahat, Erpan; Xiang, Yi; Zhang, Xiaoyan; Bekier, Michael E.; Wang, Yanzhuang
Title: GRASP depletion–mediated Golgi destruction decreases cell adhesion and migration via the reduction of a5ß1 integrin Document date: 2019_3_15
ID: rfs7m6or_25
Snippet: We then determined the total amount of protein synthesis occurring in these cells. Cells treated with control or GRASP siRNAs were labeled using 35 S-labeled methionine and cysteine for 1 h and lysed in detergent. Equal amounts of total protein were then precipitated by trichloroacetic acid (TCA), and the total amount of radioactivity incorporated was measured using a scintillation counter. As shown in Figure 7B , compared with control siRNA-trea.....
Document: We then determined the total amount of protein synthesis occurring in these cells. Cells treated with control or GRASP siRNAs were labeled using 35 S-labeled methionine and cysteine for 1 h and lysed in detergent. Equal amounts of total protein were then precipitated by trichloroacetic acid (TCA), and the total amount of radioactivity incorporated was measured using a scintillation counter. As shown in Figure 7B , compared with control siRNA-treated cells, the total protein synthesis increased when GRASPs were depleted. This result is consistent with the observation that Golgi unstacking increases cell growth, suggesting a link between protein synthesis, trafficking, posttranslational modification, and cell growth.
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