Selected article for: "culture medium and fresh medium"
Author: Zeng, Zhengyang; Zhang, Runhong; Hong, Wei; Cheng, Yuting; Wang, Huijuan; Lang, Yange; Ji, Zhenglin; Wu, Yingliang; Li, Wenxin; Xie, Youli; Cao, Zhijian
Title: Histidine-rich Modification of a Scorpion-derived Peptide Improves Bioavailability and Inhibitory Activity against HSV-1
  • Document date: 2018_1_1
    • ID: zilqyfjl_15
    Snippet: Vero cells were planted in 96-well plates (10 4 cells per well) and cultured at 37 ℃ for 24 h. The cell culture medium was then replaced with fresh medium containing candidate peptides in a series of concentrations, and the plates were incubated at 37 ℃ for another 48 h. Afterwards, 20 μl of MTT solution (5 mg/mL in phosphate buffered saline, PBS) was added to each well, and the plates were incubated at 37 ℃ for 4 h. The medium was removed.....
    Document: Vero cells were planted in 96-well plates (10 4 cells per well) and cultured at 37 ℃ for 24 h. The cell culture medium was then replaced with fresh medium containing candidate peptides in a series of concentrations, and the plates were incubated at 37 ℃ for another 48 h. Afterwards, 20 μl of MTT solution (5 mg/mL in phosphate buffered saline, PBS) was added to each well, and the plates were incubated at 37 ℃ for 4 h. The medium was removed, and 100 μl of dimethyl sulfoxide (DMSO) was added to each well, and the plates were shaken gently for 10 min at room temperature to completely dissolve the crystal purple formazan. The absorbance was then measured at 570 nm using a microplate reader (BioTek, Winooski, VT, USA).

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