Selected article for: "host cell and previous study"
Author: He, Cheng; Kong, Ling; Zhou, Lijuan; Xia, Jing; Wei, Haixia; Liu, Min; Peng, Hongjuan
Title: Host Cell Vimentin Restrains Toxoplasma gondii Invasion and Phosphorylation of Vimentin is Partially Regulated by Interaction with TgROP18
  • Document date: 2017_9_5
    • ID: z9mg639h_18
    Snippet: The results of Bi-molecular fluorescence complementation (BiFc) protein interaction screening in our previous study (data not published) indicated that T. gondii ROP18 interacts with host cell vimentin. Therefore, fluorescence resonance energy transfer (FRET) was performed to confirm this interaction. The results showed a significant interaction between TgROP18 and vimentin ( Figure 6A and B). Moreover, co-immunoprecipitation (Co-IP) experiments .....
    Document: The results of Bi-molecular fluorescence complementation (BiFc) protein interaction screening in our previous study (data not published) indicated that T. gondii ROP18 interacts with host cell vimentin. Therefore, fluorescence resonance energy transfer (FRET) was performed to confirm this interaction. The results showed a significant interaction between TgROP18 and vimentin ( Figure 6A and B). Moreover, co-immunoprecipitation (Co-IP) experiments further verified the association of TgROP18 and vimentin using both α-FLAG (fused with TgROP18) and α-vimentin antibodies for IP ( Figure 6C ). Collectively, FRET and Co-IP experiments demonstrated that host cell vimentin could bind with TgROP18.

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