Author: Girardi, Erika; Chane-Woon-Ming, Béatrice; Messmer, Mélanie; Kaukinen, Pasi; Pfeffer, Sébastien
Title: Identification of RNase L-Dependent, 3'-End-Modified, Viral Small RNAs in Sindbis Virus-Infected Mammalian Cells Document date: 2013_11_19
ID: v6uc0ijw_13
Snippet: RNase L is involved in the production of SvsRNAs. We then hypothesized that an endoribonuclease involved in the antiviral response could be implicated in SvsRNA production. RNase L is a ubiquitous, cytoplasmic, interferon-induced endonuclease that plays a key role in the cellular response to viral infections. It cleaves single-stranded RNA regions after the dinucleotides UA/UU, thereby preventing virus accumulation (28) . As such, we knocked down.....
Document: RNase L is involved in the production of SvsRNAs. We then hypothesized that an endoribonuclease involved in the antiviral response could be implicated in SvsRNA production. RNase L is a ubiquitous, cytoplasmic, interferon-induced endonuclease that plays a key role in the cellular response to viral infections. It cleaves single-stranded RNA regions after the dinucleotides UA/UU, thereby preventing virus accumulation (28) . As such, we knocked down RNase L using siRNAs, before infecting the cells with SINV. As expected, RNase L knockdown (KD) resulted in the upregulation of the SINV genome by approximately 2.5-fold ( Fig. 2A and B) . Interestingly, it also resulted in a dramatic reduction of the viral small RNAs SvsRNA-1, -2, and -3 and their precursors (Fig. 2C) .
Search related documents:
Co phrase search for related documents- antiviral response and dramatic reduction: 1
- antiviral response and interferon induce: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- antiviral response and key role: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20
- antiviral response and RNA region: 1
- antiviral response and single strand: 1, 2, 3
- cell infect and key role: 1, 2
- cell infect and single strand: 1
- cellular response and dramatic reduction: 1
- cellular response and interferon induce: 1, 2, 3
- cellular response and key role: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- cellular response and single strand: 1
- dramatic reduction and RNA region: 1
- interferon induce and key role: 1, 2
- key role and RNA region: 1, 2, 3
- key role and single strand: 1
- RNA region and single strand: 1, 2, 3, 4, 5, 6
- RNA region and single strand RNA region: 1
Co phrase search for related documents, hyperlinks ordered by date