Selected article for: "cell proliferation and EAE immunization"
Author: Lippens, Carla; Duraes, Fernanda V.; Dubrot, Juan; Brighouse, Dale; Lacroix, Mathilde; Irla, Magali; Aubry-Lachainaye, Jean-Pierre; Reith, Walter; Mandl, Judith N.; Hugues, Stéphanie
Title: IDO-orchestrated crosstalk between pDCs and Tregs inhibits autoimmunity
  • Document date: 2016_12_23
    • ID: sl8148ap_27
    Snippet: In vitro Treg suppressive assays were performed as follows. WT → WT and IDO−/− → WT chimeric mice were immunized or not with MOG35–55 + CFA. CD4+ CD25hi T cells were harvested from total skin LNs (naïve) or dLNs (day 10 after EAE immunization) and 17 000 cells were incubated with 50 000 proliferation dye-labeled 2D2 CD4+ T cells (Treg:2D2, ratio 1:3) and 50 000 LPS activated, MOG35–55 loaded, BM derived cDCs for 5 days. 2D2 T cell pr.....
    Document: In vitro Treg suppressive assays were performed as follows. WT → WT and IDO−/− → WT chimeric mice were immunized or not with MOG35–55 + CFA. CD4+ CD25hi T cells were harvested from total skin LNs (naïve) or dLNs (day 10 after EAE immunization) and 17 000 cells were incubated with 50 000 proliferation dye-labeled 2D2 CD4+ T cells (Treg:2D2, ratio 1:3) and 50 000 LPS activated, MOG35–55 loaded, BM derived cDCs for 5 days. 2D2 T cell proliferation was assessed by flow cytometry. The percentage of Treg-mediated suppression was related to 0% of proliferation (relates to 100% of suppression) in absence of cognate MOG35–55 peptide.

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