IN VITRO INTERACTION OF MOUSE HEPATITIS VIRUS AND MACROPHAGES FROM GENETICALLY RESISTANT MICE : II. BIOLOGICAL CHARACTERIZATION OF A VARIANT VIRUS MHV(C(3)H) ISOLATED FROM STOCKS OF MHV(PRI)

Shif, Ilan; Bang, Frederik B.

The appearance of a virus strain which apparently had a new host range, and which developed in association with destruction of "resistant" C3H cells, raised the question whether the destruction of C3H cells by the original MHV(PRI) preceded the emergence of the new virus or whether the emergence of the new virus was a necessary antecedent to the destruction of the cells. While the ques-tion has not yet been fully answered, one experiment has yielded some insight into the association between destruction and emergence of new virus. Serial dilutions of MHV(PRI) were inoculated onto CsH cultures. The new strain was found present in all tube cultures showing delayed destruction, while in the absence of destruction, new virus failed to appear (Table V) .